Zeiss Elyra 7 equipped with dual pco.edge sCMOS cameras, 4 Laser lines (405, 488,
561, 642) and 4 Objective lenses:
EC-Plan Neofluar 10x/0.30 M27
Plan-Aprochromat 40x/1.4 Oil DIC M27
Plan-Aprochromat 63x/1.4 Oil DIC M27
alpha Plan-Aprochromat 63x/1.46 Oil Corr M27
The Zeiss Elyra 7 system is capable of 2D and 3D wide-field microscopy, Lattice Structured
Illumination Microscopy (SIM), Total Internal Reflectance Fluorescence Microscopy
(TIRFM), and Single-Molecule Localization Microscopy (SMLM).
Lattice SIM allows very light-efficient imaging resulting in less photodamage to cells. Key features
include:
2D imaging speeds up to 255 fps
Image up to 4 channels at a time
Resolution down to 120 nm laterally, 300 nm axially
SMLM utilizes photo-switchable fluorescent dyes and proteins to induce spontaneous
鈥渂linking鈥� of single fluorophores. This allows investigation of subcellular structures
and single molecules in both fixed and live cells. Key features include:
Dual camera capability to image two different fluorophores at once
Resolution down to 20nm laterally and 50 nm axially
