The Advanced Imaging Core (AIC) provides super-resolution microscopy imaging services
through its state-of-the-art equipment and expertise.
The AIC houses super-resolution microscopy imaging equipment, enabling researchers
to capture sub-cellular structures and to identify protein localization patterns with
high accuracy in both 2D and 3D. These cutting-edge technologies minimize sample photo
damage during imaging. Live imaging on the Zeiss Elyra 7 system allows users to track
fast cellular processes such as vesicle movement, and to observe a myriad of signaling
events.Ìý
In addition to maintaining the Elyra 7, the AIC offers expert technical assistance
to investigators, including experimental design/consultation, microscope training,
assistance with imaging, and post-acquisition data analysis.Ìý
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Lattice SIM image acquired by Dr. Rachel Escue showing BPAECs stained with MitoTracker
Red CMXRos, Alexa Fluor 488 phalloidin, and DAP.
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The AIC houses light-sheet fluorescence, wide-field fluorescence, and super-resolution
flourescence microscopy imaging equipment, enabling researchers to capture subcellular
structures and to identify protein localization patterns with high accuracy in both
2D and 3D.
A LaVision Ultramicrscope II light-sheet fluorescent microscope (LSFM) is available
in the LSFM Unit and a Zeiss Elyra 7 system is available in the super resolution microscopy
(SRM) Unit.
These cutting-edge technologies minimize sample photodamage during imaging. Live imaging
on the Elyra 7 system allows users to track fast cellular processes, such as vesicle
movement, and to observe various signaling events.
In addition to maintaining these specialized instruments, the AIC offers expert technical
assistance to investigators, including experimental design/consultation, microscope
training, assistance with imaging, and post-acquisition image data analysis.
Services and Rates
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Service
Rate
Imaging with core staff assistance
$87.42/hr
Imaging by trained users
$26.52/hr for SIM or WF and $43.71/hr for SMLM
User Training
$87.42/hr with a minimum training session of 4 hours per user.
Data analysis by core staff
$87.42/hrÌý (includes image pre-processing, feature registration, and feature counting)Ìý
